Threadgold, J. and Brown, T. A. (2003). Degradation of DNA in artifically charred wheat seeds. J Archaeol Sci 30 (8). Vol 30(8), pp. 1067-1076.
 Title The title of the publication or report |
Degradation of DNA in artifically charred wheat seeds | ||||||||||
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Issue The name of the volume or issue |
J Archaeol Sci 30 (8) | ||||||||||
Series The series the publication or report is included in |
Journal of Archaeological Science | ||||||||||
Volume Volume number and part |
30 (8) | ||||||||||
Page Start/End The start and end page numbers. |
1067 - 1076 | ||||||||||
Biblio Note This is a Bibliographic record only. |
Please note that this is a bibliographic record only, as originally entered into the BIAB database. The ADS have no files for download, and unfortunately cannot advise further on where to access hard copy or digital versions. | ||||||||||
Publication Type The type of publication - report, monograph, journal article or chapter from a book |
Journal | ||||||||||
Abstract The abstract describing the content of the publication or report |
Describes artificial heating experiments that were used as a model for the events that give rise to archaeological assemblages of charred grains. Samples of Triticum aestivum L. seeds were heated at 150, 200, 225 and 250 °C for periods of up to five hours. There were two distinct patterns of weight loss during heating, one due to expulsion of water and the other involving more substantial organic changes. Blue fluorescing material, typical of the intermediate compounds in the Maillard reaction, was observed after longer periods of heating and/or exposure to the higher temperatures. At each temperature there was a sharp initial decrease in the DNA content of the seeds followed by a gradual reduction in the rate of decay. At 150 and 200 °C, the DNA decay appeared to level off with very little additional breakdown occurring after two hours, but at 225 and 250 °C the DNA content of the seeds reached undetectable levels at some point during the heating period. Polymerase chain reactions (PCRs) were used to study the degradation of the nuclear and mitochondrial genomes. PCRs were successful with seeds heated at 150 and 200 °C for all time points up to and including 5 hours. At 225 and 250 °C, both PCRs failed after shorter periods of heating. The mitochondrial product was more resistant to heating, as expected as the mitochondrial genome is present at a higher copy number than the nuclear genome. PCR products from the mitochondrial locus were cloned and sequenced to determine if heating had any effect on the accuracy of sequence information obtained from charred wheat seeds. The frequencies of sequencing errors were similar, and low, in all six samples that were studied. All aspects of seed and DNA degradation were delayed in low-oxygen samples compared with the equivalent aerobic material. | ||||||||||
Year of Publication The year the book, article or report was published |
2003 | ||||||||||
Locations Any locations covered by the publication or report. This is not the place the book or report was published. |
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Source Where the record has come from or which dataset it was orginally included in. |
BIAB
(The British & Irish Archaeological Bibliography (BIAB))
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Created Date The date the record of the pubication was first entered |
03 Feb 2004 |
