Vuissoz, A., Worobey, M., Odegaard, N., Bunce, M., Machado, C., Lynnerup, N., Peacock, E. E. and Gilbert, M. Thomas P. (2007). The survival of PCR-amplifiable DNA in cow leather. J Archaeol Sci 34 (5). Vol 34(5), pp. 823-829.

Title
Title
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Title:
The survival of PCR-amplifiable DNA in cow leather
Issue
Issue
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Issue:
J Archaeol Sci 34 (5)
Series
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Series:
Journal of Archaeological Science
Volume
Volume
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Volume:
34 (5)
Page Start/End
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Page Start/End:
823 - 829
Biblio Note
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Biblio Note
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Publication Type
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Publication Type:
Journal
Abstract
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Abstract:
The authors investigated the survival of PCR-amplifiable mitochondrial and nuclear DNA in a small number of modern and medieval bovine leather samples. The results of this preliminary investigation demonstrate that, while no nuclear DNA can be PCR-amplified from any of the specimens, mitochondrial DNA can be amplified from all samples. To investigate this contrasting pattern of DNA survival further, the authors had vegetable-tanned cow skin in their own laboratory, and directly assayed the survival of PCR-amplifiable mitochondrial and nuclear DNA at each step of the process. The results indicated that nuclear DNA is reduced to sub-amplifiable levels as a result of the tanning baths, whereas amplifiable mitochondrial DNA survives the complete process. The results suggest that old and archaeological bovine leather may represent a useful source of genetic information, although this information will most likely be limited to that which can be gained from mitochondrial DNA. Includes
Author
Author
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Author:
Annick Vuissoz
Michael Worobey
Nancy Odegaard
Michael Bunce
Carlos A Machado
N Lynnerup
Elizabeth E Peacock
M Thomas P Gilbert
Year of Publication
Year of Publication
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Year of Publication:
2007
Locations
Locations
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Subjects / Periods:
Leather (Auto Detected Subject))
Modern (Auto Detected Temporal)
Bovine Leather (Auto Detected Subject))
Archaeological Bovine Leather (Auto Detected Subject))
Medieval (Auto Detected Temporal)
Source
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Source:
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BIAB (The British & Irish Archaeological Bibliography (BIAB))
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URI: http://www.sciencedirect.com/science/journal/03054403
Created Date
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Created Date:
03 Dec 2007