Grieshaber, B. M., Osborne, D., Doubleday, A. and Kaestle, F. (2008). A pilot study into the effects of X-ray and computed tomography exposure on the amplification of DNA from bone. J Archaeol Sci 35 (3). Vol 35(3), pp. 681-687.
Title The title of the publication or report |
A pilot study into the effects of X-ray and computed tomography exposure on the amplification of DNA from bone | ||||
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Issue The name of the volume or issue |
J Archaeol Sci 35 (3) | ||||
Series The series the publication or report is included in |
Journal of Archaeological Science | ||||
Volume Volume number and part |
35 (3) | ||||
Page Start/End The start and end page numbers. |
681 - 687 | ||||
Biblio Note This is a Bibliographic record only. |
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Publication Type The type of publication - report, monograph, journal article or chapter from a book |
Journal | ||||
Abstract The abstract describing the content of the publication or report |
The research investigates the intersection of radiography and aDNA, two commonly used methods in bioarchaeology. The goal of the project was to investigate the effects of radiation on the ability to amplify DNA from bone. Bones (n = 124) from domestic pig (Sus scrofa) feet were randomly sorted into a control group and four treatment groups: (1) single exposure X-ray; (2) single exposure CT; (3) multiple exposures X-ray; and (4) multiple exposures CT. Number of PCR cycles required to amplify DNA in 100 bp, 200 bp and 400 bp segments were used as a proxy for the amount of available DNA. In the 200 bp CT sample, distal phalanges required significantly more amplification cycles than did the other bones and were removed from analysis. Results suggest that in general radiation exposure fragments DNA thereby decreasing the amount that is amplifiable. While these results are suggestive, further research is required to elucidate the degree to which radiation fragments DNA in archaeological specimens. | ||||
Year of Publication The year the book, article or report was published |
2008 | ||||
Locations Any locations covered by the publication or report. This is not the place the book or report was published. |
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Source Where the record has come from or which dataset it was orginally included in. |
BIAB
(The British & Irish Archaeological Bibliography (BIAB))
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Relations Other resources which are relevant to this publication or report |
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Created Date The date the record of the pubication was first entered |
21 Jul 2008 |