Grieshaber, B. M., Osborne, D., Doubleday, A. and Kaestle, F. (2008). A pilot study into the effects of X-ray and computed tomography exposure on the amplification of DNA from bone. J Archaeol Sci 35 (3). Vol 35(3), pp. 681-687.

Title
Title
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Title:
A pilot study into the effects of X-ray and computed tomography exposure on the amplification of DNA from bone
Issue
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Issue:
J Archaeol Sci 35 (3)
Series
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Series:
Journal of Archaeological Science
Volume
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Volume:
35 (3)
Page Start/End
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Page Start/End:
681 - 687
Biblio Note
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Journal
Abstract
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Abstract:
The research investigates the intersection of radiography and aDNA, two commonly used methods in bioarchaeology. The goal of the project was to investigate the effects of radiation on the ability to amplify DNA from bone. Bones (n = 124) from domestic pig (Sus scrofa) feet were randomly sorted into a control group and four treatment groups: (1) single exposure X-ray; (2) single exposure CT; (3) multiple exposures X-ray; and (4) multiple exposures CT. Number of PCR cycles required to amplify DNA in 100 bp, 200 bp and 400 bp segments were used as a proxy for the amount of available DNA. In the 200 bp CT sample, distal phalanges required significantly more amplification cycles than did the other bones and were removed from analysis. Results suggest that in general radiation exposure fragments DNA thereby decreasing the amount that is amplifiable. While these results are suggestive, further research is required to elucidate the degree to which radiation fragments DNA in archaeological specimens.
Author
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Author:
Britta M Grieshaber
Daniel L Osborne
Alison F Doubleday
Frederika A Kaestle
Year of Publication
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Year of Publication:
2008
Locations
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Subjects / Periods:
Pig (Auto Detected Subject))
Bone (Auto Detected Subject))
Radiography (Auto Detected Subject))
100 Bp 200 Bp (Auto Detected Temporal)
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Source:
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BIAB (The British & Irish Archaeological Bibliography (BIAB))
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URI: http://www.sciencedirect.com/science/journal/03054403
Created Date
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Created Date:
21 Jul 2008