Dissing, J., Kristinsdottir, M. and Friis, C. (2008). On the elimination of extraneous DNA in fossil human teeth with hypochlorite. J Archaeol Sci 35 (6). Vol 35(6), pp. 1445-1452.
 Title The title of the publication or report |
On the elimination of extraneous DNA in fossil human teeth with hypochlorite | |||
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Issue The name of the volume or issue |
J Archaeol Sci 35 (6) | |||
Series The series the publication or report is included in |
Journal of Archaeological Science | |||
Volume Volume number and part |
35 (6) | |||
Page Start/End The start and end page numbers. |
1445 - 1452 | |||
Biblio Note This is a Bibliographic record only. |
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Publication Type The type of publication - report, monograph, journal article or chapter from a book |
Journal | |||
Abstract The abstract describing the content of the publication or report |
Elimination of extraneous DNA in fossil specimens is important for the successful isolation and analysis of authentic DNA; this is especially true when the specimens are of human origin. Bones and teeth are commonly decontaminated with bleach containing the powerful oxidising hypochlorite ion. The procedures involve either submersion in or wiping with the chlorine agent. Using the radioactive isotope Cl36 the authors showed that submersion of fossil teeth in solutions of small ions such as Cl− or hypochlorite, ClO−, causes them to migrate right into the pulp. This may lead to the unwanted destruction of authentic DNA. However, using pairs of teeth from the remains of four ancient Europeans (1000--2000 YBP) as well as tooth and hair from an Inuit skull (>300 YBP) they provide evidence that at least some endogenous human fossil DNA survives in powdered pulp/dentin that has been submersed in 2% hypochlorite. Further, they show that powdered pulp/dentin deliberately contaminated with huge amounts of a 414 bp PCR product is effectively decontaminated by suspension in 2% hypochlorite for five minutes. Decontamination of fossil material from teeth may therefore be accomplished by a short direct action of hypochlorite on the powdered specimen rather than less controllable and less efficient external treatments of the whole specimen. Includes | |||
Year of Publication The year the book, article or report was published |
2008 | |||
Locations Any locations covered by the publication or report. This is not the place the book or report was published. |
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Source Where the record has come from or which dataset it was orginally included in. |
BIAB
(The British & Irish Archaeological Bibliography (BIAB))
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Relations Other resources which are relevant to this publication or report |
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Created Date The date the record of the pubication was first entered |
23 Jul 2008 |
