Giles, R. J. and Brown, T. A. (2008). Improved methodology for extraction and amplification of DNA from single grains of charred wheat. Journal of Archaeological Science 35 (9). Vol 35(9), pp. 2585-2588. https://doi.org/10.1016/j.jas.2008.04.011.
Title The title of the publication or report |
Improved methodology for extraction and amplification of DNA from single grains of charred wheat | ||||||
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Issue The name of the volume or issue |
Journal of Archaeological Science 35 (9) | ||||||
Series The series the publication or report is included in |
Journal of Archaeological Science | ||||||
Volume Volume number and part |
35 (9) | ||||||
Number of Pages The number of pages in the publication or report |
249 | ||||||
Page Start/End The start and end page numbers. |
2585 - 2588 | ||||||
Biblio Note This is a Bibliographic record only. |
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Publication Type The type of publication - report, monograph, journal article or chapter from a book |
Journal | ||||||
Abstract The abstract describing the content of the publication or report |
Comparisons were made between two commonly used methods for the extraction of ancient DNA from charred plant remains. Using artificially charred wheat seeds, it is shown that silica-binding is the most efficient method for extraction of DNA. Describes an improved silica-binding procedure, including pre-incubation with N-phenacylthiazolium bromide and increased washing of bound DNA, which yields amplifiable DNA from seeds heated at 200°C for up to 8 hours, conditions which promote the formation of Maillard products which often copurify with aDNA and inhibit subsequent PCRs [polymerase chain reactions]. Suggest that this method will be effective in ancient DNA extraction with most types of charred archaeobotanical material. Both cold- and hot-start PCR procedures gave good amplicon yields with extracts prepared in this way, but cold-start PCRs also resulted in synthesis of short artefact products. Addition of bovine serum albumin to PCRs, an inert carrier substance thought to enhance amplification efficiency by binding contaminants, had no advantageous effect and in fact reduced amplicon synthesis. | ||||||
Year of Publication The year the book, article or report was published |
2008 | ||||||
Locations Any locations covered by the publication or report. This is not the place the book or report was published. |
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Source Where the record has come from or which dataset it was orginally included in. |
BIAB
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Created Date The date the record of the pubication was first entered |
18 Mar 2010 |