Baker, A., Gulliver, P., Ascough, P. L., Roe, J. and Bridgeman, J. (2011). Assessing the effect of sterilization on the radiocarbon signature of freshwater dissolved organic carbon. Radiocarbon 53 (4). Vol 53(4), pp. 659-667.

Title
Title
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Title:
Assessing the effect of sterilization on the radiocarbon signature of freshwater dissolved organic carbon
Issue
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Issue:
Radiocarbon 53 (4)
Series
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Series:
Radiocarbon
Volume
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Volume:
53 (4)
Number of Pages
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Number of Pages:
182
Page Start/End
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Page Start/End:
659 - 667
Biblio Note
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Biblio Note
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Journal
Abstract
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Abstract:
Radiocarbon analysis of freshwater dissolved organic carbon (DOC) involves substantial sample pretreatment, including an initial rotary evaporation stage necessary to concentrate large volumes of freshwater sample. This may lead to a health risk from the exposure to pathogens, and there is the additional concern that the warm conditions during the rotary evaporation stage may provide ideal growing conditions for some pathogens. To remove any pathogen risk in water samples, boiling or autoclaving can be undertaken. However, to date, no studies have been undertaken to investigate whether boiling will alter the 14C signature of dissolved organic carbon. Here, the effect of sterilization on 9 contrasting river water samples is analyzed. Comparing filtered, filtered and boiled, and filtered and sterilized dissolved organic matter, it is observed that both boiling and autoclaving increases the uncertainty associated with the 14C and 13C of DOC, that the 14C and 13C changes are not unidirectional, and that they are not related to original DOC composition. Neither sterilization method is recommended unless essential, in which instance a 3σ uncertainty on 14C is recommended as is that the 13C is not considered representative of the original sample.
Author
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Author:
Andy Baker
Pauline Gulliver
Philippa L Ascough
Jessie Roe
John Bridgeman
Year of Publication
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Year of Publication:
2011
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URI: https://journals.uair.arizona.edu/index.php/radiocarbon/article/view/12347
Created Date
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Created Date:
23 Feb 2013