Pruvost, M. and Geigl, E. (2004). Real-time quantitative PCR to assess the authenticity of ancient DNA amplification. J Archaeol Sci 31 (9). Vol 31(9), pp. 1191-1197.
 Title The title of the publication or report |
Real-time quantitative PCR to assess the authenticity of ancient DNA amplification | |||||
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Issue The name of the volume or issue |
J Archaeol Sci 31 (9) | |||||
Series The series the publication or report is included in |
Journal of Archaeological Science | |||||
Volume Volume number and part |
31 (9) | |||||
Page Start/End The start and end page numbers. |
1191 - 1197 | |||||
Biblio Note This is a Bibliographic record only. |
Please note that this is a bibliographic record only, as originally entered into the BIAB database. The ADS have no files for download, and unfortunately cannot advise further on where to access hard copy or digital versions. | |||||
Publication Type The type of publication - report, monograph, journal article or chapter from a book |
Journal | |||||
Abstract The abstract describing the content of the publication or report |
Molecular analysis of DNA preserved in archaeological bone allows bypassing of certain palaeontological biases. Studies of DNA in fossils are based on the PCR amplification of the small number of molecules that have not been destroyed over time by diagenetic processes. The sensitivity of the method, the presence of polymerase inhibitors in the fossil extracts, the small number of authentic ancient DNA molecules, and their numerous chemical modifications render this powerful approach unreliable and have led to many controversies. The authors argue that real-time PCR is the method of choice to assess the initial number of genuine ancient DNA molecules in the extracts, the effect of inhibitors and the presence of low concentrations of contaminating modern target molecules, and suggest that this methodology will help to identify contaminations, to better interpret PCR results and to render ancient DNA studies more reliable. | |||||
Year of Publication The year the book, article or report was published |
2004 | |||||
Locations Any locations covered by the publication or report. This is not the place the book or report was published. |
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Source Where the record has come from or which dataset it was orginally included in. |
BIAB
(The British & Irish Archaeological Bibliography (BIAB))
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Created Date The date the record of the pubication was first entered |
06 Dec 2004 |
