| Title: |
Real-time quantitative PCR to assess the authenticity of ancient DNA amplification
|
| Issue: |
J Archaeol Sci 31 (9) |
| Series: |
Journal of Archaeological Science
|
| Volume: |
31
(9)
|
| Page Start/End: |
1191 - 1197 |
| Biblio Note |
Please note that this is a bibliographic record only, as originally entered into the BIAB database.
The ADS have no files for download, and unfortunately cannot advise further on where to access hard copy or digital versions.
|
| Publication Type: |
Journal
|
| Abstract: |
Molecular analysis of DNA preserved in archaeological bone allows bypassing of certain palaeontological biases. Studies of DNA in fossils are based on the PCR amplification of the small number of molecules that have not been destroyed over time by diagenetic processes. The sensitivity of the method, the presence of polymerase inhibitors in the fossil extracts, the small number of authentic ancient DNA molecules, and their numerous chemical modifications render this powerful approach unreliable and have led to many controversies. The authors argue that real-time PCR is the method of choice to assess the initial number of genuine ancient DNA molecules in the extracts, the effect of inhibitors and the presence of low concentrations of contaminating modern target molecules, and suggest that this methodology will help to identify contaminations, to better interpret PCR results and to render ancient DNA studies more reliable. |
| Year of Publication: |
2004
|
| Subjects / Periods: |
| Bone (Auto Detected Subject) |
|
Bones (General)
(BIAB)
|
|
Dna [Deoxyribo Nucleic Acid]
(BIAB)
|
|
| Source: |
BIAB
(The British & Irish Archaeological Bibliography (BIAB))
|
| Created Date: |
06 Dec 2004 |
